24 June 1988 Detection Of Three Rotational Correlation Times For A Rigid Asymmetric Molecule Using Frequency-Domain Fluorometry
Author Affiliations +
Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945402
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States
Abstract
We measured the frequency-response of the polarized emission of Yt-base in propylene glycol at 10°C. Data were obtained for excitation wavelengths of 290, 312 and 346 nm, for which the fundamental anisotropies are 0.05, 0.19 and 0.32, respectively. Additionally, data were obtained using CC14, to decrease the mean decay time from 9.1 to 4.2 ns. These data were analyzed globally to recover the anisotropy decay law. Three correlation times were needed to fit the data, 0.8, 3.0 and 5.6 ns, a range of only 7-fold. We believe this is the first reported detection of three correlation times for a rigid molecule.
© (1988) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
lgnacy Gryczynski, Henryk Cherek, Joseph R Lakowicz, "Detection Of Three Rotational Correlation Times For A Rigid Asymmetric Molecule Using Frequency-Domain Fluorometry", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945402; https://doi.org/10.1117/12.945402
PROCEEDINGS
8 PAGES


SHARE
Back to Top