24 June 1988 Fluorescence Studies Of Bilayers And Proteins: Critical Behavior And Genetic Engineering
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Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945375
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States
Abstract
Fluorescence decay and anisotropy studies of parinaric acid in phospholipid bilayers have demonstrated the presence of density fluctuations exhibiting critical behavior in these nominally single phase structures. The anisotropy behavior is used to extract the second and fourth rank order parameters <P2> and <P4> for comparison with models of acyl chain order. A recent study of parinaric acid in hexagonal urea inclusion complexes has demonstrated that the strongly allowed transition is polarized at an angle with respect to the chain axis. The implications of this for the interpretation of anisotropy experiments is discussed. The lysozyme from bacteriophage aq has been engineered in modified forms containing only one tryptophan residue and with substitutions near the buried tryptophan residue 138. Simple changes in the structure appear to result in large changes in the dynamics of this residue. These observations are compared with the results of molecular dynamics computations.
© (1988) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Bruce Hudson, Bruce Hudson, Anthony Ruggiero, Anthony Ruggiero, Danni Harris, Danni Harris, lain Johnson, lain Johnson, Xiu -mei Dou, Xiu -mei Dou, Thomas Novet, Thomas Novet, Lawrence McIntosh, Lawrence McIntosh, Cynthia Phillips, Cynthia Phillips, Theresa Nester, Theresa Nester, } "Fluorescence Studies Of Bilayers And Proteins: Critical Behavior And Genetic Engineering", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); doi: 10.1117/12.945375; https://doi.org/10.1117/12.945375
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