A Microcomputer Based Charge Coupled Device (CCD) Imaging System For Clinical 2D Electrophoretic Gel Analysis

Brian L. Craine; Eric R. Craine; John R. Engel; Neil T. Wemple

doi:10.1117/12.968676

27 June 1988 A Microcomputer Based Charge Coupled Device (CCD) Imaging System For Clinical 2D Electrophoretic Gel Analysis

Brian L. Craine, Eric R. Craine, John R. Engel, Neil T. Wemple

Author Affiliations +

Proceedings Volume 0914, Medical Imaging II; (1988) https://doi.org/10.1117/12.968676
Event: Medical Imaging II, 1988, Newport Beach, CA, United States

Abstract

We have developed a prototype microcomputer based gel reader for the digitization and semi-automatic analysis of two dimensional polyacrylamide electrophoretic gels. The system uses a 2D charge coupled device (CCD) detector and operates as a peripheral to an IBM PC XT/AT type host computer. The system incorporates a comprehensive software package which lends itself to highly structured clinical applications as well as a wide range of image processing functions for use in the research laboratory. The system is undergoing further development in order to arrive at a comprehensive, low cost, early disease screening device. Electrophoretic gel samples of protein components obtained from subject patients are digitized and searched for anomalous features. These features are located using a digital reference library of disease related protein features and a probability of detection of the disease related protein is computed. The system then presents the physician with a report advising him of the results of the analysis. We describe the hardware and software system and present results of a sample gel analysis.

Citation Download Citation

Brian L. Craine, Eric R. Craine, John R. Engel, and Neil T. Wemple "A Microcomputer Based Charge Coupled Device (CCD) Imaging System For Clinical 2D Electrophoretic Gel Analysis", Proc. SPIE 0914, Medical Imaging II, (27 June 1988); https://doi.org/10.1117/12.968676

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