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16 January 1989 Review Of Optical Methods In Immunosensing
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Proceedings Volume 0954, Optical Testing and Metrology II; (1989)
Event: SPIE International Symposium on Optical Engineering and Industrial Sensing for Advance Manufacturing Technologies, 1988, Dearborn, MI, United States
Currently within decentralised clinical laboratories there is a need for simple and rapid techniques to detect antigens and antibodies of diagnostic significance. The interaction between antigen and antibody molecules may be extremely specific as a consequence of binding geometries and is a principal phenomenon of antibody-based chemical sensors, i.e. immunosensors. Direct optical monitoring of this interaction is an attractive alternative in the development of immunosensors, because optical sensors can be very sensitive, small in size, safe, and cheap. This paper reviews immunosensors based on optical sensing techniques including such methods like light reflectance (the Brewster angle reflectometry, surface plasmon resonance), and evanescent wave fluorescence. The basic principles and the current state-of-the art are discussed with emphasis on sensitivity, measurement range, shortcomings and problems to be solved. Some comments on miniaturisation and future developments will also be given.
© (1989) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Janusz W. Sadowski "Review Of Optical Methods In Immunosensing", Proc. SPIE 0954, Optical Testing and Metrology II, (16 January 1989);

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