Photochemical Targeting Of Phagocytic Trabecular Meshwork Cells Using Chlorin E6 Coupled Microspheres

M. A. Latina; P. H. Kobsa; S. L. Rakestraw; E. A. Crean; T. Hasan; M. L. Yarmush

doi:10.1117/12.960182

17 March 1989 Photochemical Targeting Of Phagocytic Trabecular Meshwork Cells Using Chlorin E6 Coupled Microspheres

M. A. Latina, P. H. Kobsa, S. L. Rakestraw, E. A. Crean, T. Hasan, M. L. Yarmush

Proceedings Volume 0997, Advances in Photochemotherapy; (1989) https://doi.org/10.1117/12.960182
Event: O-E/Fiber LASE '88, 1988, Boston, MA, United States

Abstract

We have investigated a novel and efficient delivery system utilizing photosensitizer-coupled-latex microspheres to photochemically target and kill phagocytic trabecular meshwork (TM) cells. TM cells are the most actively phagocytic cells within the anterior chamber of the eye and are located within an optically accessible discrete band. This delivery system, along with the property of cell photocytosis, will achieve double selectivity by combining preferential localization of the photosensitizer to the target cells with spatial localization of illumination on the target cells. All experiments were performed with preconfluent bovine TM cells, 3rd to 4th passage, plated in 15 mm wells. Chlorin e6 monoethylene diamine monoamide was conjugated to the surface of 1.0 Am MX Duke Scientific fluorescent latex microspheres. Spectroscopic analysis revealed an average of 1.3 x 10 -17 moles of chlorin e6 per microsphere. TM cells were incubated for 18 hours with 5 x 10 7 microspheres/ml in MEM with 10% FCS, washed with MEM, and irradiated through fresh media using an argon-pumped dye laser emitting .2 W at 660 nm. A dose-survival study indicated that energy doses of 10 J/cm2 or greater resulted in greater than 95% cell death as determined by ethidium bromide exclusion. Cell death could be demonstrated as early as 4 hours post-irradiation. TM cells incubated with a solution of chlorin e6 at a concentration equal to that conjugated to the microspheres showed no cell death. Unirradiated controls also showed no cell death.

Citation Download Citation

M. A. Latina, P. H. Kobsa, S. L. Rakestraw, E. A. Crean, T. Hasan, and M. L. Yarmush "Photochemical Targeting Of Phagocytic Trabecular Meshwork Cells Using Chlorin E6 Coupled Microspheres", Proc. SPIE 0997, Advances in Photochemotherapy, (17 March 1989); https://doi.org/10.1117/12.960182

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