9 December 2016 Enzyme catalyzed optofluidic biolaser for sensitive ion concentration detection
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Abstract
The enzyme horseradish peroxidase (HRP) has been extensively used in biochemistry for its ability to amplify a weak signal. By using HRP catalyzed substrate as the gain medium, we demonstrate sensitive ion concentration detection based on the optofluidic laser. The enzyme catalyzed reaction occurs in bulk solution inside a Fabry-Perot laser cavity, where the colorless, non-fluorescent 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) substrate is oxidized to produce highly fluorescent resorufin. Laser emission is achieved when pumped with the second harmonic wave of a Q-switched YAG laser. Further, we use sulfide anion (S2-) as an example to investigate the sensing performance of enzyme catalyzed optofluidic laser. The laser onset time difference between the sample to be tested and the reference is set to be the sensing output. Thanks to the amplification effects of both the enzymatic reaction and laser emission, we achieve a detection limit of 10 nM and a dynamic range of 3 orders of magnitude.
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Chaoyang Gong, Yuan Gong, Maung Kyaw Khaing Oo, Yu Wu, Yunjiang Rao, Xudong Fan, "Enzyme catalyzed optofluidic biolaser for sensitive ion concentration detection", Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100131S (9 December 2016); doi: 10.1117/12.2244668; https://doi.org/10.1117/12.2244668
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