Fluorescence enhancement of photoswitchable metal ion sensors

Georgina Sylvia; Sabrina Heng; Andrew D. Abell

doi:10.1117/12.2244656

9 December 2016 Fluorescence enhancement of photoswitchable metal ion sensors

Georgina Sylvia, Sabrina Heng, Andrew D. Abell

Proceedings Volume 10013, SPIE BioPhotonics Australasia; 100131X (2016) https://doi.org/10.1117/12.2244656
Event: SPIE BioPhotonics Australasia, 2016, Adelaide, Australia

Abstract

Spiropyran-based fluorescence sensors are an ideal target for intracellular metal ion sensing, due to their biocompatibility, red emission frequency and photo-controlled reversible analyte binding for continuous signal monitoring. However, increasing the brightness of spiropyran-based sensors would extend their sensing capability for live-cell imaging. In this work we look to enhance the fluorescence of spiropyran-based sensors, by incorporating an additional fluorophore into the sensor design. We report a 5-membered monoazacrown bearing spiropyran with metal ion specificity, modified to incorporate the pyrene fluorophore. The effect of N-indole pyrene modification on the behavior of the spiropyran molecule is explored, with absorbance and fluorescence emission characterization. This first generation sensor provides an insight into fluorescence-enhancement of spiropyran molecules.

Citation Download Citation

Georgina Sylvia, Sabrina Heng, and Andrew D. Abell "Fluorescence enhancement of photoswitchable metal ion sensors", Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100131X (9 December 2016); https://doi.org/10.1117/12.2244656

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