9 December 2016 Fluorescence enhancement of photoswitchable metal ion sensors
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Spiropyran-based fluorescence sensors are an ideal target for intracellular metal ion sensing, due to their biocompatibility, red emission frequency and photo-controlled reversible analyte binding for continuous signal monitoring. However, increasing the brightness of spiropyran-based sensors would extend their sensing capability for live-cell imaging. In this work we look to enhance the fluorescence of spiropyran-based sensors, by incorporating an additional fluorophore into the sensor design. We report a 5-membered monoazacrown bearing spiropyran with metal ion specificity, modified to incorporate the pyrene fluorophore. The effect of N-indole pyrene modification on the behavior of the spiropyran molecule is explored, with absorbance and fluorescence emission characterization. This first generation sensor provides an insight into fluorescence-enhancement of spiropyran molecules.
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Georgina Sylvia, Georgina Sylvia, Sabrina Heng, Sabrina Heng, Andrew D. Abell, Andrew D. Abell, } "Fluorescence enhancement of photoswitchable metal ion sensors", Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100131X (9 December 2016); doi: 10.1117/12.2244656; https://doi.org/10.1117/12.2244656

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