9 December 2016 PScan 1.0: flexible software framework for polygon based multiphoton microscopy
Author Affiliations +
Multiphoton laser scanning microscopes exhibit highly localized nonlinear optical excitation and are powerful instruments for in-vivo deep tissue imaging. Customized multiphoton microscopy has a significantly superior performance for in-vivo imaging because of precise control over the scanning and detection system. To date, there have been several flexible software platforms catered to custom built microscopy systems i.e. ScanImage, HelioScan, MicroManager, that perform at imaging speeds of 30-100fps. In this paper, we describe a flexible software framework for high speed imaging systems capable of operating from 5 fps to 1600 fps. The software is based on the MATLAB image processing toolbox. It has the capability to communicate directly with a high performing imaging card (Matrox Solios eA/XA), thus retaining high speed acquisition. The program is also designed to communicate with LabVIEW and Fiji for instrument control and image processing. Pscan 1.0 can handle high imaging rates and contains sufficient flexibility for users to adapt to their high speed imaging systems.
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yongxiao Li, Yongxiao Li, Woei Ming Lee, Woei Ming Lee, } "PScan 1.0: flexible software framework for polygon based multiphoton microscopy", Proc. SPIE 10013, SPIE BioPhotonics Australasia, 1001333 (9 December 2016); doi: 10.1117/12.2242954; https://doi.org/10.1117/12.2242954


High speed multiphoton imaging
Proceedings of SPIE (December 09 2016)
Inspection of microchip mounting tolerances by 3D vision
Proceedings of SPIE (February 25 2008)
Multiphoton analysis of normal and diseased livers
Proceedings of SPIE (March 30 2005)
In vivo multiphoton imaging of bile duct ligation
Proceedings of SPIE (February 15 2008)

Back to Top