31 October 2016 Detection and classification of ebola on microfluidic chips
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Abstract
Point-of-care testing (POCT) for an infectious diseases is the prerequisite to control of the disease and limitation of its spread. A microfluidic chip for detection and classification of four strains of Ebola virus was developed and evaluated. This assay was based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and specific primers for Ebola Zaire virus, Ebola Sudan virus, Ebola Tai Forest virus and Ebola Bundibugyo virus were designed. The sensitivity of the microfluidic chip was under 103 copies per milliliter, as determined by ten repeated tests. This assay is unique in its ability to enable diagnosis of the Ebola infections and simultaneous typing of Ebola virus on a single chip. It offers short reaction time, ease of use and high specificity. These features should enable POCT in remote area during outbreaks of Ebola virus.
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Xue Lin, Xue Lin, Xiangyu Jin, Xiangyu Jin, Yunqian Fan, Yunqian Fan, Qin Huang, Qin Huang, Yue Kou, Yue Kou, Guo Zu, Guo Zu, Shiguang Huang, Shiguang Huang, Xiaosheng Liu, Xiaosheng Liu, Guoliang Huang, Guoliang Huang, } "Detection and classification of ebola on microfluidic chips", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100240B (31 October 2016); doi: 10.1117/12.2246154; https://doi.org/10.1117/12.2246154
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