31 October 2016 Evaluation of free radical scavenging and anti-oxidative capacity of polydatin-nanostructured lipid carriers
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Cellular damage induced by free-radicals like reactive oxygen species has been implicated in several diseases. 2, 2-azobis(2-amidino-propane) dihydrochloride(AAPH) generates two potent ROS capable of inducing lipid peroxidation: alkoxy radical(RO-) and peroxy radical (ROO-). These radicals are similar to those that are physiologically active and thus might initiate a cascade of intracellular toxic events leading to oxidation, lipid peroxidation, DNA damage and subsequent cell death. Hence naturally anti-oxidant play a vital role in combating these conditions. In this study, polydatin loaded nanostructured lipid carriers (Pol-NLC) was prepared by hot melting and then high pressure homogenization technique. The effects of Pol-NLC on free radical scavenging and anti-oxidative capacity is investigated. The particle size and zeta potential of Pol-NLC were 113.9 ± 1.1 nm and −16.3 1 ± 0.27 mV, respectively. By free radical scavenging assays, the IC50 value of Pol-NLC were 28.71, 9.83 μg/mL with DPPH, ABTS assay respectively, and 0.143 mg ferrous sulfate/1 mg Pol-NLC with FRAP assay. These results indicated that the antioxidant properties of Pol-NLC hold great potential used as an alternative to more toxic synthetic anti-oxidants as an additive in food, cosmetic and pharmaceutical preparations for the oxidative diseases treatment.
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Xiang-Ping Meng, Xiang-Ping Meng, Fan Shi, Fan Shi, Hai-Jie Li, Hai-Jie Li, Li-De Yin, Li-De Yin, Yi-Fei Wang, Yi-Fei Wang, Zhi-ping Wang, Zhi-ping Wang, Tong-sheng Chen, Tong-sheng Chen, } "Evaluation of free radical scavenging and anti-oxidative capacity of polydatin-nanostructured lipid carriers", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100241B (31 October 2016); doi: 10.1117/12.2245532; https://doi.org/10.1117/12.2245532

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