Identification of the boundary between normal brain tissue and ischemia region using two-photon excitation fluorescence microscopy

Huiping Du; Shu Wang; Xingfu Wang; Xiaoqin Zhu; Shuangmu Zhuo; Jianxin Chen

doi:10.1117/12.2245399

31 October 2016 Identification of the boundary between normal brain tissue and ischemia region using two-photon excitation fluorescence microscopy

Huiping Du, Shu Wang, Xingfu Wang, Xiaoqin Zhu, Shuangmu Zhuo, Jianxin Chen

Author Affiliations +

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 1002422 (2016) https://doi.org/10.1117/12.2245399
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

Ischemic stroke is one of the common neurological diseases, and it is becoming the leading causes of death and permanent disability around the world. Early and accurate identification of the potentially salvageable boundary region of ischemia brain tissues may enable selection of the most appropriate candidates for early stroke therapies. In this work, TPEF microscopy was used to image the microstructures of normal brain tissues, ischemia regions and the boundary region between normal and ischemia brain tissues. The ischemia brain tissues from Sprague-Dawley (SD) rats were subjected to 6 hours of middle cerebral artery occlusion (MCAO). Our study demonstrates that TPEF microscopy has the ability to not only reveal the morphological changes of the neurons but also identify the boundary between normal brain tissue and ischemia region, which correspond well to the hematoxylin and eosin (H and E) stained images. With the development of miniaturized TPEF microscope imaging devices, TPEF microscopy can be developed into an effectively diagnostic and monitoring tool for cerebral ischemia.

Citation Download Citation

Huiping Du, Shu Wang, Xingfu Wang, Xiaoqin Zhu, Shuangmu Zhuo, and Jianxin Chen "Identification of the boundary between normal brain tissue and ischemia region using two-photon excitation fluorescence microscopy", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 1002422 (31 October 2016); https://doi.org/10.1117/12.2245399

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Ischemia

Brain

Tissues

Microscopy

Neuroimaging

Neurons

Luminescence

Show All Keywords

Keywords/Phrases

Search In:

Publication Years