31 October 2016 Identification of the boundary between normal brain tissue and ischemia region using two-photon excitation fluorescence microscopy
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Abstract
Ischemic stroke is one of the common neurological diseases, and it is becoming the leading causes of death and permanent disability around the world. Early and accurate identification of the potentially salvageable boundary region of ischemia brain tissues may enable selection of the most appropriate candidates for early stroke therapies. In this work, TPEF microscopy was used to image the microstructures of normal brain tissues, ischemia regions and the boundary region between normal and ischemia brain tissues. The ischemia brain tissues from Sprague-Dawley (SD) rats were subjected to 6 hours of middle cerebral artery occlusion (MCAO). Our study demonstrates that TPEF microscopy has the ability to not only reveal the morphological changes of the neurons but also identify the boundary between normal brain tissue and ischemia region, which correspond well to the hematoxylin and eosin (H and E) stained images. With the development of miniaturized TPEF microscope imaging devices, TPEF microscopy can be developed into an effectively diagnostic and monitoring tool for cerebral ischemia.
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Huiping Du, Shu Wang, Xingfu Wang, Xiaoqin Zhu, Shuangmu Zhuo, Jianxin Chen, "Identification of the boundary between normal brain tissue and ischemia region using two-photon excitation fluorescence microscopy", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 1002422 (31 October 2016); doi: 10.1117/12.2245399; https://doi.org/10.1117/12.2245399
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