31 October 2016 Quantification of collagen distributions in rat hyaline and fibro cartilages based on second harmonic generation imaging
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Abstract
Hyaline cartilage is a semitransparent tissue composed of proteoglycan and thicker type II collagen fibers, while fibro cartilage large bundles of type I collagen besides other territorial matrix and chondrocytes. It is reported that the meniscus (fibro cartilage) has a greater capacity to regenerate and close a wound compared to articular cartilage (hyaline cartilage). And fibro cartilage often replaces the type II collagen-rich hyaline following trauma, leading to scar tissue that is composed of rigid type I collagen. The visualization and quantification of the collagen fibrillar meshwork is important for understanding the role of fibril reorganization during the healing process and how different types of cartilage contribute to wound closure. In this study, second harmonic generation (SHG) microscope was applied to image the articular and meniscus cartilage, and textural analysis were developed to quantify the collagen distribution. High-resolution images were achieved based on the SHG signal from collagen within fresh specimens, and detailed observations of tissue morphology and microstructural distribution were obtained without shrinkage or distortion. Textural analysis of SHG images was performed to confirm that collagen in fibrocartilage showed significantly coarser compared to collagen in hyaline cartilage (p < 0.01). Our results show that each type of cartilage has different structural features, which may significantly contribute to pathology when damaged. Our findings demonstrate that SHG microscopy holds potential as a clinically relevant diagnostic tool for imaging degenerative tissues or assessing wound repair following cartilage injury.
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Xiaoqin Zhu, Xiaoqin Zhu, Chenxi Liao, Chenxi Liao, Zhenyu Wang, Zhenyu Wang, Shuangmu Zhuo, Shuangmu Zhuo, Wenge Liu, Wenge Liu, Jianxin Chen, Jianxin Chen, } "Quantification of collagen distributions in rat hyaline and fibro cartilages based on second harmonic generation imaging", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 1002424 (31 October 2016); doi: 10.1117/12.2245516; https://doi.org/10.1117/12.2245516
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