31 October 2016 Quantitative optical biomarkers of lung cancer based intrinsic two-photon excited fluorescence signal
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Abstract
Alterations in the elastic fibers have been implicated in lung cancer. However, the label-free, microscopic imaging of elastic fibers in situ remains a major challenge. Here, we present the use of intrinsic two-photon excited fluorescence (TPEF) signal as a novel means for quantification of the elastic fibers in intact fresh human lung tissues. We obtained the TPEF images of elastic fibers from ex vivo the human lung tissues. We found that three features, including the elastic fibers area, the elastic fibers orientation, the elastic fibers structure, provide the quantitative identification of lung cancer and the direct visual cues for cancer versus non-cancer areas. These results suggest that the TPEF signal can be used as the label-free optical biomarkers for rapid clinical lung diagnosis and instant image-guided surgery.
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Jingwen Li, Jingwen Li, Zhenlin Zhan, Zhenlin Zhan, Hongxin Lin, Hongxin Lin, Ning Zuo, Ning Zuo, Xiaoqin Zhu, Xiaoqin Zhu, Shusen Xie, Shusen Xie, Jianxin Chen, Jianxin Chen, Shuangmu Zhuo, Shuangmu Zhuo, } "Quantitative optical biomarkers of lung cancer based intrinsic two-photon excited fluorescence signal", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100242I (31 October 2016); doi: 10.1117/12.2245730; https://doi.org/10.1117/12.2245730
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