31 October 2016 Application study of transport intensity equation in quantitative phase reconstruction
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In order to improve detection speed and accuracy of biological cells, a quantitative non-interference optical phase recovery method is proposed in commercial microscope, taking the red blood cells as the classical phase objects. Three bright field micrographs were collected in the experiment. Utilizing the transport intensity equation (TIE), the quantitative phase distributions of red blood cell are gained and agree well with the previous optical phase models. Analysis shows that the resolution of introduced system reaches sub-micron. This method not only quickly gives quantitative phase distribution of cells, but also measures a large number of cells simultaneously. So it is potential in the use of real-time observing and quantitative analyzing of cells in vivo.
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Xiaojun Song, Xiaojun Song, Wei Cheng, Wei Cheng, Chunjuan Wei, Chunjuan Wei, Liang Xue, Liang Xue, Weijing Liu, Weijing Liu, Baodan Bai, Baodan Bai, Fenghong Chu, Fenghong Chu, } "Application study of transport intensity equation in quantitative phase reconstruction", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100243F (31 October 2016); doi: 10.1117/12.2246139; https://doi.org/10.1117/12.2246139

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