31 October 2016 Blood vessel damage correlated with irradiance for in vivo vascular targeted photodynamic therapy
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Abstract
Vascular targeted photodynamic therapy (V-PDT) has been widely utilized for the prevention or treatment of vascular-related diseases, including age-related macular degeneration, port-wine stains and prostate cancer. In order to quantitative assessment the blood vessel damage during V-PDT, nude mice were implanted with Titanium dorsal skin window chambers for in vivo V-PDT studies. For treatments, various irradiances including 50, 75, 100 and 200 mW/cm2 provided by a 532 nm semiconductor laser were performed with the same total light dose of 30 J/cm2 after the mice were intravenously injection of Rose Bengal for 25 mg/Kg body weight. Laser speckle imaging and microscope were used to monitor blood flow dynamics and vessel constriction during and after V-PDT, respectively. The V-PDT induced vessel damages between different groups were compared. The results show that significant difference in blood vessel damage was found between the lower irradiances (50, 75 and 100 mW/cm2) and higher irradiance (200 mW/cm2), and the blood vessel damage induced by V-PDT is positively correlated with irradiance. This study implies that the optimization of irradiance is required for enhancing V-PDT therapeutic efficiency.
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Jinde Zhang, Jinde Zhang, Zou Tan, Zou Tan, Xiangyu Niu, Xiangyu Niu, Linsheng Lin, Linsheng Lin, Huiyun Lin, Huiyun Lin, Buhong Li, Buhong Li, } "Blood vessel damage correlated with irradiance for in vivo vascular targeted photodynamic therapy", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100244N (31 October 2016); doi: 10.1117/12.2246530; https://doi.org/10.1117/12.2246530
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