31 October 2016 Non-invasive optical detection of HBV based on serum surface-enhanced Raman spectroscopy
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Abstract
An optical method of surface-enhanced Raman spectroscopy (SERS) was developed for non-invasive detection of hepatitis B surface virus (HBV). Hepatitis B virus surface antigen (HBsAg) is an established serological marker that is routinely used for the diagnosis of acute or chronic hepatitis B virus(HBV) infection. Utilizing SERS to analyze blood serum for detecting HBV has not been reported in previous literature. SERS measurements were performed on two groups of serum samples: one group for 50 HBV patients and the other group for 50 healthy volunteers. Blood serum samples are collected from healthy control subjects and patients diagnosed with HBV. Furthermore, principal components analysis (PCA) combined with linear discriminant analysis (LDA) were employed to differentiate HBV patients from healthy volunteer and achieved sensitivity of 80.0% and specificity of 74.0%. This exploratory work demonstrates that SERS serum analysis combined with PCA-LDA has tremendous potential for the non-invasive detection of HBV.
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Zuci Zheng, Zuci Zheng, Qiwen Wang, Qiwen Wang, Cuncheng Weng, Cuncheng Weng, Xueliang Lin, Xueliang Lin, Yao Lin, Yao Lin, Shangyuan Feng, Shangyuan Feng, } "Non-invasive optical detection of HBV based on serum surface-enhanced Raman spectroscopy", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100244S (31 October 2016); doi: 10.1117/12.2247671; https://doi.org/10.1117/12.2247671
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