14 November 2016 Amphiphilic block copolymer-based photonic platform towards efficient protein detection
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Abstract
The development of a low complexity fiber optic based protein sensor by functionalizing the surface of silica optical fibers using block copolymers having both hydrophobic poly(methyl methacrylate) (PMMA) and hydrophilic poly[2- (dimethylamino)ethyl methacrylate] (PDMAEMA) blocks is presented here. The amphiphilic thiol-functionalized PMMA117-b-P(DMAEMA17-st-TEMA2) and vinyl-sulfone PMMA117-b-P(DMAEMA17-st-VSTEMA2) block copolymers designed and synthesized in this work contain a cationic hydrophilic PDMAEMA block that can electrostatically bind selected oppositely charged proteins and also appropriate functional groups for reversible or non-reversible protein binding, respectively, leading to a refractive index change of the overlayer and hence, enabling the sensing. The developed PMMA117-b-PDMAEMA16-based platform has been evaluated for bovine serum albumin (BSA) sensing, exhibiting linear response to detected BSA concentrations.
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Afroditi Petropoulou, Afroditi Petropoulou, Thomas J. Gibson, Thomas J. Gibson, Efrosyni Themistou, Efrosyni Themistou, Stergios Pispas, Stergios Pispas, Christos Riziotis, Christos Riziotis, } "Amphiphilic block copolymer-based photonic platform towards efficient protein detection", Proc. SPIE 10025, Advanced Sensor Systems and Applications VII, 100250M (14 November 2016); doi: 10.1117/12.2246506; https://doi.org/10.1117/12.2246506
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