Bioinformatic investigation of the role of ubiquitins in cucumber flower morphogenesis

Magdalena Pawełkowicz; Paweł Osipowski; Michał Wojcieszek; Cezary Kowalczuk; Wojciech Pląder; Zbigniew Przybecki

doi:10.1117/12.2249108

28 September 2016 Bioinformatic investigation of the role of ubiquitins in cucumber flower morphogenesis

Magdalena Pawełkowicz, Paweł Osipowski, Michał Wojcieszek, Cezary Kowalczuk, Wojciech Pląder, Zbigniew Przybecki

Proceedings Volume 10031, Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2016; 100312Y (2016) https://doi.org/10.1117/12.2249108
Event: Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2016, 2016, Wilga, Poland

Abstract

Three cDNA clones were used to screen cucumber genome in order to find genes and proteins. Functional annotation reveals that they are correlated with ubiquitination pathways. Various bioinformatics tools were used to screen and check protein sequences features such as: the presence of specific domains, transmembrane regions, cleavage site and cellular placement. The computational analysis for promotor region shows many binding sites for transcription factors, which could regulate the expression of genes. In order to check gene expression levels in developing flower buds of monoecious (B10) and gynoecious (2gg) cucumber lines, the real – time PCR technique was applied. The expression was checked for the whole buds and only for the 3^rd and 4^th whorls of bud when generative organ are form which were obtained by Laser Capture Microdissection (LCM) technique.

Citation Download Citation

Magdalena Pawełkowicz, Paweł Osipowski, Michał Wojcieszek, Cezary Kowalczuk, Wojciech Pląder, and Zbigniew Przybecki "Bioinformatic investigation of the role of ubiquitins in cucumber flower morphogenesis", Proc. SPIE 10031, Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2016, 100312Y (28 September 2016); https://doi.org/10.1117/12.2249108

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