CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10037 > Article
Open Access Presentation
19 April 2017 In-vivo multiphoton microscopy (MPM) of laser-induced optical breakdown (LIOB) in human skin (Conference Presentation)
Mihaela Balu, Griffin Lentsch, Dorota Korta M.D., Karsten Konig, Kristen M. Kelly, Bruce J. Tromberg, Christopher B. Zachary M.D.
Author Affiliations +
Proceedings Volume 10037, Photonics in Dermatology and Plastic Surgery; 100370F (2017) https://doi.org/10.1117/12.2253566
Event: SPIE BiOS, 2017, San Francisco, California, United States
Abstract
We use a multiphoton microscopy (MPM)-based clinical microscope (MPTflex, JenLab, Germany) to describe changes in human skin following treatment with a fractional non-ablative laser (PicoWay, Candela). The treatment was based on a fractionated picosecond Nd:YAG laser (1064 and 532nm, 3mJ and 1.5mJ (no attenuation), respectively maximum energy/pulse, 100 microbeams/6mmx6mm). Improvements in skin appearance resulting from treatment with this laser have been noted but optimizing the efficacy depends on a thorough understanding of the specific skin response to treatment. MPM is a nonlinear laser scanning microscopy technique that features sub-cellular resolution and label-free molecular contrast. MPM contrast in skin is derived from second-harmonic generation of collagen and two-photon excited fluorescence of NADH/FAD+, elastin, keratin, melanin. In this pilot study, two areas on the arm of a volunteer (skin type II) were treated with the picoWay laser (1064nm, 3mJ; 532nm, 1.5mJ; 1pass). The skin response to treatment was imaged in-vivo at 8 time points over the following 4 weeks. MPM revealed micro-injuries present in epidermis. Damaged individual cells were distinguished after 3h and 24h from treatment with both wavelengths. Pigmented cells were particularly damaged in the process, suggesting that melanin is the main absorber and the primary target for laser induced optical breakdown. At later time points, clusters of cellular necrotic debris were imaged across the treated epidermis. These results represent the groundwork for future longitudinal studies on expanded number of subjects to understand the response to treatment in different skin types at different laser parameters, critical factors in optimizing treatment outcomes.
Conference Presentation

View presentation recording on the SPIE Digital Library: http://dx.doi.org/10.1117/12.2253566.5369740743001
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Mihaela Balu, Griffin Lentsch, Dorota Korta M.D., Karsten Konig, Kristen M. Kelly, Bruce J. Tromberg, and Christopher B. Zachary M.D. "In-vivo multiphoton microscopy (MPM) of laser-induced optical breakdown (LIOB) in human skin (Conference Presentation)", Proc. SPIE 10037, Photonics in Dermatology and Plastic Surgery, 100370F (19 April 2017); https://doi.org/10.1117/12.2253566
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Skin
Laser optics
In vivo imaging
Multiphoton microscopy
Tissue optics
Medical laser equipment
Microscopes
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top