Targeted sections in either XY or XZ plane with dual-axes confocal endomicroscope (Conference Presentation)

Gaoming Li; Haijun Li; Xiyu Duan; Quan Zhou; Juan Zhou; Kenn R. Oldham; Thomas D. Wang M.D.

doi:10.1117/12.2251633

19 April 2017 Targeted sections in either XY or XZ plane with dual-axes confocal endomicroscope (Conference Presentation)

Gaoming Li, Haijun Li, Xiyu Duan, Quan Zhou, Juan Zhou, Kenn R. Oldham, Thomas D. Wang M.D.

Author Affiliations +

Proceedings Volume 10040, Endoscopic Microscopy XII; 100400X (2017) https://doi.org/10.1117/12.2251633
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We demonstrate a dual axes confocal architecture, which can be used to collect horizontal(XY-plane) or vertical cross-sectional(XZ-plane) images for tissue. This scanner head is 5.5mm in outer diameter(OD), and integrates a 3D MEMS scanner with a compact chip size of 3.2×2.9mm2. To realize the miniaturization, there are some obstacles of the small size of 3D MEMS scanner, MEMS wire bundle, the air pressure effect for MEMS motion, the processing of parabolic mirror, and optical alignment to come over. In our probe, separation mechanical structure for optical alignment was adopted and a step shape MEMS holder was designed to deal with the difficult of MEMS wire bundle. Peptides have been demonstrated tremendous potential for in vivo use to detect colonic dysplasia. This class of in vivo molecular probe can be labeled with near-infrared (NIR) dyes for visualizing the full depth of the epithelium in small animals. To confirm our probe performance, we take use of USAF 1951 resolution target to test its lateral and axial resolution. It has lateral and axial resolution of 2.49um and 4.98um, respectively. When we collect the fluorescence imaging of colon, it shows that the field of view are 1000um×1000um (horizontal) and 1000um×430um (vertical). The horizontal and vertical cross-sectional images of fresh mouse colonic mucosa demonstrate imaging performance with this miniature instrument.

Conference Presentation

Citation Download Citation

Gaoming Li, Haijun Li, Xiyu Duan, Quan Zhou, Juan Zhou, Kenn R. Oldham, and Thomas D. Wang M.D. "Targeted sections in either XY or XZ plane with dual-axes confocal endomicroscope (Conference Presentation)", Proc. SPIE 10040, Endoscopic Microscopy XII, 100400X (19 April 2017); https://doi.org/10.1117/12.2251633

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KEYWORDS

Microelectromechanical systems

3D scanning

Confocal microscopy

Laser scanners

In vivo imaging

Optical alignment

Head

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