8 February 2017 Optical coherence microscopy with extended focus for in vivo embryonic imaging
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Optical coherence microscopy (OCM) has unique advantages of high-resolution volumetric imaging without relying on exogenous labels or dyes. It combines the coherence-gated depth discrimination of optical coherence tomography (OCT) with the high lateral resolution of confocal microscopy, offering an excellent balance between the resolutions and imaging depth. However, as the lateral resolution becomes higher, the imaging depth of OCM decreases and its three-dimensional imaging capability is greatly degraded. To overcome this limitation, we used amplitude apodization to create quasi-Bessel beam illumination in order to extend the depth of focus. The lateral and axial resolutions of our OCM system were measured to be 1.6 μm and 2.9 μm in tissue. The imaging depth was extended by ~3.0X (~100 μm) beyond that of the standard Gaussian beam OCM. Using zebrafish embryos as a test system, we demonstrate extendedfocus OCM for structural imaging studies, which revealed the detailed anatomy deep in embryos.
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Yu Chen, Yu Chen, Jeff Fingler, Jeff Fingler, Scott E. Fraser, Scott E. Fraser, } "Optical coherence microscopy with extended focus for in vivo embryonic imaging", Proc. SPIE 10043, Diagnosis and Treatment of Diseases in the Breast and Reproductive System, 1004310 (8 February 2017); doi: 10.1117/12.2253306; https://doi.org/10.1117/12.2253306

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