8 February 2017 Assessing the dynamic biofilm removal of sulfonated phenolics using CP-OCT
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Proceedings Volume 10044, Lasers in Dentistry XXIII; 1004409 (2017) https://doi.org/10.1117/12.2254624
Event: SPIE BiOS, 2017, San Francisco, California, United States
Abstract
Examining the physical mechanisms related to biofilm removal of sulfonated phenolics (SP) is difficult using conventional microscopy techniques. A custom flow cell system integrated with a real time cross polarization optical coherence tomography system investigated the dynamic speed of biofilm removal when oral multi-species biofilms are exposed to SP under sheer stress. The Near infrared 1310-nm CP-OCT system non-destructively imaged fluid immersed oral biofilms at nearly 30 frames/s. This dynamic imaging was able to determine the cohesive and adhesion related disruption of SP on oral biofilms adhering to tooth like surfaces. For multi-species biofilms that are initially grown without the presence of sucrose, the disruption of biofilms on saliva coated hydroxyapatite (HA) is dominated as a adhesive failure at the HA-biofilm interface. For multi-species biofilms that are grown in the presence of sucrose, the disruption is dominated by cohesive disruption followed by adhesive failure. This novel CP-OCT flow cell assay has the potential to examine rapid interactions between anti-biofilm agents and tooth like surfaces.
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K. Englund, K. Englund, J. Nikrad, J. Nikrad, R. Jones, R. Jones, } "Assessing the dynamic biofilm removal of sulfonated phenolics using CP-OCT", Proc. SPIE 10044, Lasers in Dentistry XXIII, 1004409 (8 February 2017); doi: 10.1117/12.2254624; https://doi.org/10.1117/12.2254624
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