Noninvasive label-free monitoring of cosmetics and pharmaceuticals in human skin using nonlinear optical microscopy (Conference Presentation)

Sam Osseiran; Hequn Wang; Conor L. Evans

doi:10.1117/12.2253390

19 April 2017 Noninvasive label-free monitoring of cosmetics and pharmaceuticals in human skin using nonlinear optical microscopy (Conference Presentation)

Sam Osseiran, Hequn Wang, Conor L. Evans

Author Affiliations +

Proceedings Volume 10046, Visualizing and Quantifying Drug Distribution in Tissue; 1004605 (2017) https://doi.org/10.1117/12.2253390
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Over the past decade, nonlinear optical microscopy has seen a dramatic rise in its use in research settings due to its noninvasiveness, enhanced penetration depth, intrinsic optical sectioning, and the ability to probe chemical compounds with molecular specificity without exogenous contrast agents. Nonlinear optical techniques including two-photon excitation fluorescence (2PEF), fluorescence lifetime imaging microscopy (FLIM), second harmonic generation (SHG), coherent anti-Stokes and stimulated Raman scattering (CARS and SRS, respectively), as well as transient and sum frequency absorption (TA and SFA, respectively), have been widely used to explore the physiology and microanatomy of skin. Recently, these modalities have shed light on dermal processes that could not have otherwise been observed, including the spatiotemporal monitoring of cosmetics and pharmaceuticals. However, a challenge quickly arises when studying such chemicals in a dermatological context: many exogenous compounds have optical signatures that can interfere with the signals that would otherwise be acquired from intact skin. For example, oily solvents exhibit strong signals when probing CH2 vibrations with CARS/SRS; chemical sun filters appear bright in 2PEF microscopy; and darkly colored compounds readily absorb light across a broad spectrum, producing strong TA/SFA signals. Thus, this discussion will first focus on the molecular contrast in skin that can be probed using the aforementioned nonlinear optical techniques. This will be followed by an overview of strategies that take advantage of the exogenous compounds’ optical signatures to probe spatiotemporal dynamics while preserving endogenous information from skin.

Conference Presentation

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Sam Osseiran, Hequn Wang, and Conor L. Evans "Noninvasive label-free monitoring of cosmetics and pharmaceuticals in human skin using nonlinear optical microscopy (Conference Presentation)", Proc. SPIE 10046, Visualizing and Quantifying Drug Distribution in Tissue, 1004605 (19 April 2017); https://doi.org/10.1117/12.2253390

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KEYWORDS

Skin

Microscopy

Optical microscopy

Transient nonlinear optics

Tissue optics

Fluorescence lifetime imaging

Chemical compounds

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