Singlet oxygen explicit dosimetry to predict long-term local tumor control for BPD-mediated photodynamic therapy

Michele M. Kim; Rozhin Penjweini; Yi Hong Ong; Timothy C. Zhu

doi:10.1117/12.2250435

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13 February 2017 Singlet oxygen explicit dosimetry to predict long-term local tumor control for BPD-mediated photodynamic therapy

Michele M. Kim, Rozhin Penjweini, Yi Hong Ong, Timothy C. Zhu

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Proceedings Volume 10047, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI; 100470X (2017) https://doi.org/10.1117/12.2250435
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Photodynamic therapy (PDT) is a well-established treatment modality for cancer and other malignant diseases; however, quantities such as light fluence, photosensitizer photobleaching rate, and PDT dose do not fully account for all of the dynamic interactions between the key components involved. In particular, fluence rate (Φ) effects are not accounted for, which has a large effect on the oxygen consumption rate. In this preclinical study, reacted singlet oxygen [¹O₂]_rx was investigated as a dosimetric quantity for PDT outcome. The ability of [¹O₂]_rx to predict the long-term local tumor control rate (LCR) for BPD-mediated PDT was examined. Mice bearing radioactivelyinduced fibrosarcoma (RIF) tumors were treated with different in-air fluences (250, 300, and 350 J/cm²) and in-air ϕ (75, 100, and150 mW/cm2) with a BPD dose of 1 mg/kg and a drug-light interval of 3 hours. Treatment was delivered with a collimated laser beam of 1 cm diameter at 690 nm. Explicit dosimetry of initial tissue oxygen concentration, tissue optical properties, and BPD concentration was used to calculate [¹O₂]_rx. Φ was calculated for the treatment volume based on Monte-Carlo simulations and measured tissue optical properties. Kaplan-Meier analyses for LCR were done for an endpoint of tumor volume ≤ 100 mm³ using four dose metrics: light fluence, photosensitizer photobleaching rate, PDT dose, and [¹O₂]_rx. PDT dose was defined as the product of the timeintegral of photosensitizer concentration and Φ at a 3 mm tumor depth. Preliminary studies show that [¹O₂]_rx better correlates with LCR and is an effective dosimetric quantity that can predict treatment outcome.

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Michele M. Kim, Rozhin Penjweini, Yi Hong Ong, and Timothy C. Zhu "Singlet oxygen explicit dosimetry to predict long-term local tumor control for BPD-mediated photodynamic therapy", Proc. SPIE 10047, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI, 100470X (13 February 2017); https://doi.org/10.1117/12.2250435

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