Imaging human brain cyto- and myelo-architecture with quantitative OCT (Conference Presentation)

David A. Boas; Hui Wang; Ender Konukoglu; Bruce Fischl; Sava Sakadzic; Caroline V. Magnain

doi:10.1117/12.2254827

19 April 2017 Imaging human brain cyto- and myelo-architecture with quantitative OCT (Conference Presentation)

David A. Boas, Hui Wang, Ender Konukoglu, Bruce Fischl, Sava Sakadzic, Caroline V. Magnain

Proceedings Volume 10051, Neural Imaging and Sensing; 100510C (2017) https://doi.org/10.1117/12.2254827
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

No current imaging technology allows us to directly and without significant distortion visualize the microscopic and defining anatomical features of the human brain. Ex vivo histological techniques can yield exquisite planar images, but the cutting, mounting and staining that are required components of this type of imaging induce distortions that are different for each slice, introducing cross-slice differences that prohibit true 3D analysis. We are overcoming this issue by utilizing Optical Coherence Tomography (OCT) with the goal to image whole human brain cytoarchitectural and laminar properties with potentially 3.5 µm resolution in block-face without the need for exogenous staining. From the intrinsic scattering contrast of the brain tissue, OCT gives us images that are comparable to Nissl stains, but without the distortions introduced in standard histology as the OCT images are acquired from the block face prior to slicing and thus without the need for subsequent staining and mounting. We have shown that laminar and cytoarchitectural properties of the brain can be characterized with OCT just as well as with Nissl staining. We will present our recent advances to improve the axial resolution while maintaining contrast; improvements afforded by speckle reduction procedures; and efforts to obtain quantitative maps of the optical scattering coefficient, an intrinsic property of the tissue.

Conference Presentation

Citation Download Citation

David A. Boas, Hui Wang, Ender Konukoglu, Bruce Fischl, Sava Sakadzic, and Caroline V. Magnain "Imaging human brain cyto- and myelo-architecture with quantitative OCT (Conference Presentation)", Proc. SPIE 10051, Neural Imaging and Sensing, 100510C (19 April 2017); https://doi.org/10.1117/12.2254827

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Optical coherence tomography

Brain

Neuroimaging

Distortion

Scattering

Tissue optics

3D image processing

Show All Keywords

Keywords/Phrases

Search In:

Publication Years