Simultaneous two-photon imaging of cerebral oxygenation and capillary blood flow in atherosclerotic mice

Xuecong Lu; Baoqiang Li; Mohammad Moeini; Frédéric Lesage

doi:10.1117/12.2251437

8 February 2017 Simultaneous two-photon imaging of cerebral oxygenation and capillary blood flow in atherosclerotic mice

Xuecong Lu, Baoqiang Li, Mohammad Moeini, Frédéric Lesage

Author Affiliations +

Proceedings Volume 10051, Neural Imaging and Sensing; 100510O (2017) https://doi.org/10.1117/12.2251437
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Gradual changes in brain microvasculature and cerebral capillary blood flow occurring with atherosclerosis may significantly contribute to cognition decline due to their role in brain tissue oxygenation. However, previous stud- ies of the relationship between cerebral capillary blood flow and brain tissue oxygenation are limited. This study aimed to investigate vascular and concomitant changes in brain tissue pO₂ with atherosclerosis. Experiments in young healthy C57B1/6 mice (n=6 , WT), young atherosclerotic mice (n=6 , ATX Y) and old atherosclerotic mice (n=6 , ATX O) were performed imaging on the left sensory-motor cortex at resting state under urethane (1.5 g/kg) anesthesia using two-photon fluorescence microscopy. The results showed that pO₂ around capillaries, correlated with red blood cell (RBC) flux, increased with atherosclerosis.

Conference Presentation

Citation Download Citation

Xuecong Lu, Baoqiang Li, Mohammad Moeini, and Frédéric Lesage "Simultaneous two-photon imaging of cerebral oxygenation and capillary blood flow in atherosclerotic mice", Proc. SPIE 10051, Neural Imaging and Sensing, 100510O (8 February 2017); https://doi.org/10.1117/12.2251437

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available