8 February 2017 Rapid imaging of mammalian brain slices with a compact light sheet fluorescent microscope
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Proceedings Volume 10051, Neural Imaging and Sensing; 100510T (2017) https://doi.org/10.1117/12.2251227
Event: SPIE BiOS, 2017, San Francisco, California, United States
Abstract
Light sheet fluorescent microscopy is able to provide high acquisition speed and high contrast images, as well as the low photo-bleaching and photo-damage brought to the sample. Here we describe a compact setup design optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We report this prototype instrument is capable of rapid imaging wide area of the dendritic or axonal arbor of a dye-filled neuron in hippocampal slice. We also show several applications of this compact light sheet fluorescent microscope, to demonstrate that our approach offers a powerful functionality to the neuroscience community that is not achievable with traditional imaging methods.
Conference Presentation
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Zhengyi Yang, Peter Haslehurst, Suzanne Scott, Nigel Emptage, Kishan Dholakia, "Rapid imaging of mammalian brain slices with a compact light sheet fluorescent microscope", Proc. SPIE 10051, Neural Imaging and Sensing, 100510T (8 February 2017); doi: 10.1117/12.2251227; https://doi.org/10.1117/12.2251227
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