A pH-sensitive red fluorescent protein compatible with hydrophobic resin embedding

Wenyan Guo; Yadong Gang; Xiuli Liu; Hongfu Zhou; Shaoqun Zeng

doi:10.1117/12.2250844

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8 February 2017 A pH-sensitive red fluorescent protein compatible with hydrophobic resin embedding

Wenyan Guo, Yadong Gang, Xiuli Liu, Hongfu Zhou, Shaoqun Zeng

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Proceedings Volume 10051, Neural Imaging and Sensing; 1005119 (2017) https://doi.org/10.1117/12.2250844
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

pH sensitive fluorescent proteins enabling chemical reactivation in resin are useful tools for fluorescence microimaging. EYFP or EGFP improved from GFP in jellyfish are good for such applications. For simultaneous two-color imaging, a suitable red fluorescent protein is of urgent need. Here a pH sensitive red fluorescent protein, pHuji, is selected and verified to be compatible with hydrophobic resin embedding and thus may be promising for dual-colour chemical reactivation imaging in conjunction with EGFP or EYFP.

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Wenyan Guo, Yadong Gang, Xiuli Liu, Hongfu Zhou, and Shaoqun Zeng "A pH-sensitive red fluorescent protein compatible with hydrophobic resin embedding", Proc. SPIE 10051, Neural Imaging and Sensing, 1005119 (8 February 2017); https://doi.org/10.1117/12.2250844

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