8 February 2017 Super-duper chemiluminescent proteins applicable to wide range of bioimaging
Author Affiliations +
Proceedings Volume 10052, Optogenetics and Optical Manipulation; 1005202 (2017) https://doi.org/10.1117/12.2249770
Event: SPIE BiOS, 2017, San Francisco, California, United States
We report five new spectral variants of bright luminescent protein made by concatenation of the brightest luciferase, NanoLuc, with various color hues of fluorescent proteins. These proteins, which we call enhanced Nano-lanterns (eNLs), allow five-color live-cell imaging without external light illumination as well as detection of single molecules. Furthermore, eNL-based Ca2+ indicators could be used to image long-term Ca2+ dynamics in iPS-derived cardiomyocytes.
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Kazushi Suzuki, Kazushi Suzuki, Takeharu Nagai, Takeharu Nagai, } "Super-duper chemiluminescent proteins applicable to wide range of bioimaging", Proc. SPIE 10052, Optogenetics and Optical Manipulation, 1005202 (8 February 2017); doi: 10.1117/12.2249770; https://doi.org/10.1117/12.2249770

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