3 March 2017 Multi-scale silica structures for improved point of care detection
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Abstract
The need for sensitive, portable diagnostic tests at the point of care persists. We report on a simple method to obtain improved detection of biomolecules by a two-fold mechanism. Silica (SiO2) is coated on pre-stressed thermoplastic shrink-wrap film. When the film retracts, the resulting micro- and nanostructures yield far-field fluorescence signal enhancements over their planar or wrinkled counterparts. Because the film shrinks by 95% in surface area, there is also a 20x concentration effect. The SiO2 structured substrate is therefore used for improved detection of labeled proteins and DNA hybridization via both fluorescent and bright field. Through optical characterization studies, we attribute the fluorescence signal enhancements of ~100x to increased surface density and light scattering from the rough SiO2 structures. Combining with our open channel self-wicking microfluidics, we can achieve extremely low cost yet sensitive point of care diagnostics.
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Sophia Lin, Sophia Lin, Lancy Lin, Lancy Lin, Eunbyul Cho, Eunbyul Cho, Gaston A. O. Pezzani, Gaston A. O. Pezzani, Michelle Khine, Michelle Khine, } "Multi-scale silica structures for improved point of care detection", Proc. SPIE 10055, Optics and Biophotonics in Low-Resource Settings III, 100550S (3 March 2017); doi: 10.1117/12.2258655; https://doi.org/10.1117/12.2258655
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