15 March 2017 Label-free hyperspectral dark-field microscopy for quantitative scatter imaging
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Abstract
A hyperspectral dark-field microscope has been developed for imaging spatially distributed diffuse reflectance spectra from light-scattering samples. In this report, quantitative scatter spectroscopy is demonstrated with a uniform scattering phantom, namely a solution of polystyrene microspheres. A Monte Carlo-based inverse model was used to calculate the reduced scattering coefficients of samples of different microsphere concentrations from wavelength-dependent backscattered signal measured by the dark-field microscope. The results are compared to the measurement results from a NIST double-integrating sphere system for validation. Ongoing efforts involve quantitative mapping of scattering and absorption coefficients in samples with spatially heterogeneous optical properties.
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Philip Cheney, David McClatchy, Stephen Kanick, Paul Lemaillet, David Allen, Daniel Samarov, Brian Pogue, Jeeseong Hwang, "Label-free hyperspectral dark-field microscopy for quantitative scatter imaging", Proc. SPIE 10056, Design and Quality for Biomedical Technologies X, 1005602 (15 March 2017); doi: 10.1117/12.2263336; https://doi.org/10.1117/12.2263336
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