15 March 2017 Label-free hyperspectral dark-field microscopy for quantitative scatter imaging
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A hyperspectral dark-field microscope has been developed for imaging spatially distributed diffuse reflectance spectra from light-scattering samples. In this report, quantitative scatter spectroscopy is demonstrated with a uniform scattering phantom, namely a solution of polystyrene microspheres. A Monte Carlo-based inverse model was used to calculate the reduced scattering coefficients of samples of different microsphere concentrations from wavelength-dependent backscattered signal measured by the dark-field microscope. The results are compared to the measurement results from a NIST double-integrating sphere system for validation. Ongoing efforts involve quantitative mapping of scattering and absorption coefficients in samples with spatially heterogeneous optical properties.
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Philip Cheney, Philip Cheney, David McClatchy, David McClatchy, Stephen Kanick, Stephen Kanick, Paul Lemaillet, Paul Lemaillet, David Allen, David Allen, Daniel Samarov, Daniel Samarov, Brian Pogue, Brian Pogue, Jeeseong Hwang, Jeeseong Hwang, } "Label-free hyperspectral dark-field microscopy for quantitative scatter imaging", Proc. SPIE 10056, Design and Quality for Biomedical Technologies X, 1005602 (15 March 2017); doi: 10.1117/12.2263336; https://doi.org/10.1117/12.2263336

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