21 March 2017 Investigation of burn effect on skin using simultaneous Raman-Brillouin spectroscopy, and fluorescence microspectroscopy
Author Affiliations +
Proceedings Volume 10057, Multimodal Biomedical Imaging XII; 1005705 (2017) https://doi.org/10.1117/12.2253474
Event: SPIE BiOS, 2017, San Francisco, California, United States
Burns are thermal injuries that can completely damage or at least compromise the protective function of skin, and affect the ability of tissues to manage moisture. Burn-damaged tissues exhibit lower elasticity than healthy tissues, due to significantly reduced water concentrations and plasma retention. Current methods for determining burn intensity are limited to visual inspection, and potential hospital x-ray examination. We present a unique confocal microscope capable of measuring Raman and Brillouin spectra simultaneously, with concurrent fluorescence investigation from a single spatial location, and demonstrate application by investigating and characterizing the properties of burn-afflicted tissue on chicken skin model. Raman and Brillouin scattering offer complementary information about a material's chemical and mechanical structure, while fluorescence can serve as a useful diagnostic indicator and imaging tool. The developed instrument has the potential for very diverse analytical applications in basic biomedical science and biomedical diagnostics and imaging.
Conference Presentation
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Zachary Coker, Zachary Coker, Zhaokai Meng, Zhaokai Meng, Maria Troyanova-Wood, Maria Troyanova-Wood, Andrew Traverso, Andrew Traverso, Charles Ballmann, Charles Ballmann, Georgi Petrov, Georgi Petrov, Bennett L. Ibey, Bennett L. Ibey, Vladislav Yakovlev, Vladislav Yakovlev, } "Investigation of burn effect on skin using simultaneous Raman-Brillouin spectroscopy, and fluorescence microspectroscopy", Proc. SPIE 10057, Multimodal Biomedical Imaging XII, 1005705 (21 March 2017); doi: 10.1117/12.2253474; https://doi.org/10.1117/12.2253474

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