15 February 2017 Multimodal fluorescence and photoacoustic microscopy in the frequency domain
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Proceedings Volume 10057, Multimodal Biomedical Imaging XII; 1005707 (2017) https://doi.org/10.1117/12.2251796
Event: SPIE BiOS, 2017, San Francisco, California, United States
In this paper a multimodal optical resolution microscope in frequency domain is introduced. Fluorescence and photoacoustic signals are generated simultaneously using an amplitude modulated diode laser. The resulting signals are recorded by a lock-in amplifier. By scanning the sample, two-dimensional plots of the fluorescence and photoacoustic signals can be generated. In this paper, the signal-to-noise ratios of amplitude modulated signals are compared to those expected for pulsed excitation under the assumption that the maximum permissible exposure limits are fulfilled. Furthermore, it is described how to determine the excited state life-times from the measured frequency responses of the fluorescence or photoacoustic signals, respectively.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Gregor Langer, Gregor Langer, Bianca Buchegger, Bianca Buchegger, Jaroslaw Jacak, Jaroslaw Jacak, Karoline Pfeffer, Karoline Pfeffer, Thomas A. Klar, Thomas A. Klar, Thomas Berer, Thomas Berer, } "Multimodal fluorescence and photoacoustic microscopy in the frequency domain", Proc. SPIE 10057, Multimodal Biomedical Imaging XII, 1005707 (15 February 2017); doi: 10.1117/12.2251796; https://doi.org/10.1117/12.2251796

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