Combining Raman spectroscopy and digital holographic microscopy for label-free classification of human immune cells (Conference Presentation)

Naomi McReynolds; Fiona G. M. Cooke; Mingzhou Chen; Simon J. Powis; Kishan Dholakia

doi:10.1117/12.2250505

19 April 2017 Combining Raman spectroscopy and digital holographic microscopy for label-free classification of human immune cells (Conference Presentation)

Naomi McReynolds, Fiona G. M. Cooke, Mingzhou Chen, Simon J. Powis, Kishan Dholakia

Author Affiliations +

Proceedings Volume 10057, Multimodal Biomedical Imaging XII; 100570A (2017) https://doi.org/10.1117/12.2250505
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Moving towards label-free techniques for cell identification is essential for many clinical and research applications. Raman spectroscopy and digital holographic microscopy (DHM) are both label-free, non-destructive optical techniques capable of providing complimentary information. We demonstrate a multi-modal system which may simultaneously take Raman spectra and DHM images to provide both a molecular and a morphological description of our sample. In this study we use Raman spectroscopy and DHM to discriminate between three immune cell populations CD4+ T cells, B cells, and monocytes, which together comprise key functional immune cell subsets in immune responses to invading pathogens. Various parameters that may be used to describe the phase images are also examined such as pixel value histograms or texture analysis. Using our system it is possible to consider each technique individually or in combination. Principal component analysis is used on the data set to discriminate between cell types and leave-one-out cross-validation is used to estimate the efficiency of our method. Raman spectroscopy provides specific chemical information but requires relatively long acquisition times, combining this with a faster modality such as DHM could help achieve faster throughput rates. The combination of these two complimentary optical techniques provides a wealth of information for cell characterisation which is a step towards achieving label free technology for the identification of human immune cells.

Conference Presentation

Citation Download Citation

Naomi McReynolds, Fiona G. M. Cooke, Mingzhou Chen, Simon J. Powis, and Kishan Dholakia "Combining Raman spectroscopy and digital holographic microscopy for label-free classification of human immune cells (Conference Presentation)", Proc. SPIE 10057, Multimodal Biomedical Imaging XII, 100570A (19 April 2017); https://doi.org/10.1117/12.2250505

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KEYWORDS

Digital holography

Raman spectroscopy

Holography

Microscopy

Chemical analysis

Astronomy

Physics

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