17 February 2017 Wide-field fluorescence diffuse optical tomography with epi-illumination of sinusoidal pattern
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Abstract
We present a wide-field fluorescence tomography with epi-illumination of sinusoidal pattern. In this scheme, a DMD projector is employed as a spatial light modulator to generate independently wide-field sinusoidal illumination patterns at varying spatial frequencies on a sample, and then the emitted photons at the sample surface were captured with a EM-CCD camera. This method results in a significantly reduced number of the optical field measurements as compared to the point-source-scanning ones and thereby achieves a fast data acquisition that is desired for a dynamic imaging application. Fluorescence yield images are reconstructed using the normalized-Born formulated inversion of the diffusion model. Experimental reconstructions are presented on a phantom embedding the fluorescent targets and compared for a combination of the multiply frequencies. The results validate the ability of the method to determine the target relative depth and quantification with an increasing accuracy.
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Tongxin Li, Feng Gao, Weiting Chen, Caixia Qi, Panpan Yan, Huijuan Zhao, "Wide-field fluorescence diffuse optical tomography with epi-illumination of sinusoidal pattern", Proc. SPIE 10059, Optical Tomography and Spectroscopy of Tissue XII, 100591Y (17 February 2017); doi: 10.1117/12.2250474; https://doi.org/10.1117/12.2250474
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