Observation of chest tumor using diffuse optical spectroscopy: time-varying Indocyanine green concentration in rabbit model

Yikeun Kim; Sehun Kim; Sungwon Kim; Haeyoung Lee; Chulho Oak; Yeh-Chan Ahn

doi:10.1117/12.2251665

17 February 2017 Observation of chest tumor using diffuse optical spectroscopy: time-varying Indocyanine green concentration in rabbit model

Yikeun Kim, Sehun Kim, Sungwon Kim, Haeyoung Lee, Chulho Oak, Yeh-Chan Ahn

Author Affiliations +

Proceedings Volume 10059, Optical Tomography and Spectroscopy of Tissue XII; 1005923 (2017) https://doi.org/10.1117/12.2251665
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

This experiment was conducted by using the diffuse optical spectroscopy based on near-infrared light. The near-infrared light in the water window was used to see the change of molecular concentration in the living tissue. The experiment subject was New Zealand rabbits weighing 3 ± 0.3 kg. VX2 tumor cells were injected into the inside of the chest wall of rabbits. The concentration of indocyanine green (ICG) has been observed once every three days, after the size of the pleural tumor grew up over 1cm. We used five different wavelengths (732, 758, 805, 840, and 880 nm) with known ICG spectrum. The distance between light source and detector probes was fixed by 1 cm. The probes were placed on the skin right above the tumor with an aid of laparoscope. ICG was injected into rabbits via ear vein. The diffused light was measured through the tumor with time course using a spectrometer. These measured data enabled us to observe the change of ICG concentration in real time with respect to the baseline without ICG. ICG was present longer in tumor compared to normal tissue. This phenomenon is thought to be due to the excessive angiogenesis in the tumor tissue. Since this method can be applied to other cases easily, it is thought that there is a possibility of cancer screening with less cost and simple equipment.

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Yikeun Kim, Sehun Kim, Sungwon Kim, Haeyoung Lee, Chulho Oak, and Yeh-Chan Ahn "Observation of chest tumor using diffuse optical spectroscopy: time-varying Indocyanine green concentration in rabbit model", Proc. SPIE 10059, Optical Tomography and Spectroscopy of Tissue XII, 1005923 (17 February 2017); https://doi.org/10.1117/12.2251665

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KEYWORDS

Tumors

Tissues

Diffuse optical spectroscopy

Indocyanine green

Absorption

Cancer

Laparoscopy

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