17 February 2017 Oral cancer detection based on fluorescence polarization of blood plasma at excitation wavelength 405 nm
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Abstract
During metabolism the metabolites such as hormones, proteins and enzymes were released in to the blood stream by the cells. These metabolites reflect any change that occurs due to any disturbances in normal metabolic function of the human system. This was well observed with the altered spectral signatures observed with fluorescence spectroscopic technique. Previously many have reported on the significance of native fluorescence spectroscopic method in the diagnosis of cancer. As fluorescence spectroscopy is sensitive and simple, it has complementary techniques such as excitation-emission matrix, synchronous and polarization. The fluorescence polarization measurement provides details about any association or binding reactions and denaturing effects that occurs due to change in the micro environment of cells and tissues. In this study, we have made an attempt in the diagnosis of oral cancer at 405 nm excitation using fluorescence polarization measurement. The fluorescence anisotropic values calculated from polarized fluorescence spectral data of normal and oral cancer subjects yielded a good accuracy when analyzed with linear discriminant analysis based artificial neural network. The results will be discussed in detail.
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Rekha Pachaiappan, Rekha Pachaiappan, Aruna Prakasarao, Aruna Prakasarao, Yuvaraj Manoharan, Yuvaraj Manoharan, Koteeswaran Dornadula, Koteeswaran Dornadula, Ganesan Singaravelu, Ganesan Singaravelu, } "Oral cancer detection based on fluorescence polarization of blood plasma at excitation wavelength 405 nm", Proc. SPIE 10060, Optical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 100601D (17 February 2017); doi: 10.1117/12.2255718; https://doi.org/10.1117/12.2255718
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