28 February 2017 Rapid structuring of proteins on filter paper using lithography
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Abstract
Microfluidic paper based analytical devices (μPADs) are simple and cost efficient and can be used everywhere without the need for a high standard laboratory for obtaining a readout. These devices are thus especially suited for the developing world or crisis regions. To fabricate a bioanalytical test, certain biomolecules like proteins or antibodies have to be attached to paper strips. Common immobilization methods often rely on non-covalent, unoriented attachment which leads to reduced bioactivity of the immobilized species. Specific Immobilization of biomolecules on surfaces still poses a great challenge to biochemical research and applications.

We propose a method for the specific immobilization of biomolecules on functionalized filter paper using a maskless projection lithography setup. The paper was functionalized either by applying an adhesive protein coating or by covalent attachment of methacrylate groups. Fluorescently labelled biomolecules were attached by exploiting the formation of radical species upon bleaching of the fluorophore. A custom made maskless photo-lithography setup and a low cost approach were used to produce microscale biomolecule greyscale patterns. Protein patterns were visualized by fluorescence, enzyme patterns were tested for bioactivity by substrate conversion with colorimetric readout.

This method enables the creation of complex, highly specific bioactive protein patterns and greatly facilitates the production of μPADs.
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Tobias M. Nargang, Tobias M. Nargang, Frederik Kotz, Frederik Kotz, Nico Keller, Nico Keller, Dorothea Helmer, Dorothea Helmer, Bastian E. Rapp, Bastian E. Rapp, } "Rapid structuring of proteins on filter paper using lithography", Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 1006104 (28 February 2017); doi: 10.1117/12.2252116; https://doi.org/10.1117/12.2252116
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