25 January 2018 Assessment of geometry in 2D immune systems using high accuracy laser-based bioprinting techniques (Conference Presentation) (Withdrawal Notice)
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Publisher’s Note: This conference presentation, originally published on 4/19/17, was withdrawn per author request.

The immune system is a very complex system that comprises a network of genetic and signaling pathways subtending a network of interacting cells. The location of the cells in a network, along with the gene products they interact with, rules the behavior of the immune system. Therefore, there is a great interest in understanding properly the role of a cell in such networks to increase our knowledge of the immune system response. In order to acquire a better understanding of these processes, cell printing with high spatial resolution emerges as one of the promising approaches to organize cells in two and three-dimensional patterns to enable the study the geometry influence in these interactions. In particular, laser assisted bio-printing techniques using sub-nanosecond laser sources have better characteristics for application in this field, mainly due to its higher spatial resolution, cell viability percentage and process automation. This work presents laser assisted bio-printing of antigen-presenting cells (APCs) in two-dimensional geometries, placing cellular components on a matrix previously generated on demand, permitting to test the molecular interactions between APCs and lymphocytes; as well as the generation of two-dimensional structures designed ad hoc in order to study the mechanisms of mobilization of immune system cells. The use of laser assisted bio-printing, along with APCs and lymphocytes emulate the structure of different niches of the immune system so that we can analyse functional requirement of these interaction.

View presentation recording on the SPIE Digital Library: http://dx.doi.org/10.1117/12.2253622.5371973290001

© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Sara Lauzurica, Sara Lauzurica, Andrés Márquez, Andrés Márquez, Carlos Molpeceres, Carlos Molpeceres, Laura Notario, Laura Notario, Miguel Gómez-Fontela, Miguel Gómez-Fontela, Pilar Lauzurica, Pilar Lauzurica, "Assessment of geometry in 2D immune systems using high accuracy laser-based bioprinting techniques (Conference Presentation) (Withdrawal Notice)", Proc. SPIE 10062, Optical Interactions with Tissue and Cells XXVIII, 100620Q (25 January 2018); doi: 10.1117/12.2253622; https://doi.org/10.1117/12.2253622

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