3 March 2017 Noise sources in Raman spectroscopy of biological objects
Author Affiliations +
Abstract
We present an overview of noise sources deteriorating the quality of the recorded biological Raman spectra and the ability to determine the specimen composition. The acquired Raman spectra exhibit intense additive noise components or drifts because of low intensity of the scattered light. Therefore we have to apply expensive or bulky measurement setups to limit their inherent noise or to apply additional signal processing to reduce random components after recording the spectra (e.g., Savitzky-Golay filtering, polynomial approximation, denoising by empirical mode decomposition). We present noise sources generated in detectors of Raman scattered photon stream. We consider the methods of background noise reduction by increasing averaging time when the background noise comprises of white noise and 1/f noise components. We consider how the background noise reduces the accuracy of chemical compounds estimation using Raman spectra and prediction model based on linear (e.g., Principal Component Analysis) or nonlinear (e.g., Support Vector Machine) methods. Finally, we give some remarks about synchronous detection and background noise reduction.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
J. Smulko, J. Smulko, M. S. Wróbel, M. S. Wróbel, } "Noise sources in Raman spectroscopy of biological objects", Proc. SPIE 10063, Dynamics and Fluctuations in Biomedical Photonics XIV, 100630Q (3 March 2017); doi: 10.1117/12.2254807; https://doi.org/10.1117/12.2254807
PROCEEDINGS
7 PAGES


SHARE
Back to Top