23 March 2017 Real-time photoacoustic flow cytography and photothermolysis of single circulating melanoma cells in vivo
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Abstract
Metastasis is responsible for as many as 90% of cancer-related deaths, and the deadliest skin cancer, melanoma, has a high propensity for metastasis. Since hematogenous spread of circulating tumor cells (CTCs) is cancer’s main route of metastasis, detecting and destroying CTCs can impede metastasis and improve patients’ prognoses. Extensive studies employing exogenous agents to detect tumor-specific biomarkers and guide therapeutics to CTCs have achieved promising results, but biosafety remains a critical concern. Taking another approach, physical detection and destruction of CTCs is a safer way to evaluate and reduce metastasis risks. Melanoma cells strongly express melanosomes, providing a striking absorption contrast with the blood background in the red to near-infrared spectrum. Exploiting this intrinsic optical absorption contrast of circulating melanoma cells, we coupled dual-wavelength photoacoustic flow cytography with a nanosecond-pulsed laser killing mechanism that specifically targets melanoma CTCs. We have successfully achieved in vivo label-free imaging of rare single CTCs and CTC clusters in mice. Further, the photoacoustic signal from a CTC immediately hardware-triggers a lethal pinpoint laser irradiation that lyses it on the spot in a thermally confined manner. Our technology can facilitate early inhibition of metastasis by clearing circulating tumor cells from vasculature.
Conference Presentation
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Yun He, Yun He, Lidai Wang, Lidai Wang, Junhui Shi, Junhui Shi, Junjie Yao, Junjie Yao, Lei Li, Lei Li, Ruiying Zhang, Ruiying Zhang, Chih-Hsien Huang, Chih-Hsien Huang, Jun Zou, Jun Zou, Lihong V. Wang, Lihong V. Wang, } "Real-time photoacoustic flow cytography and photothermolysis of single circulating melanoma cells in vivo", Proc. SPIE 10064, Photons Plus Ultrasound: Imaging and Sensing 2017, 100641E (23 March 2017); doi: 10.1117/12.2255068; https://doi.org/10.1117/12.2255068
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