Dynamical optical imaging monocytes/macrophages migration and activation in contact hypersensitivity (Conference Presentation)

Zhihong Zhang

doi:10.1117/12.2251088

19 April 2017 Dynamical optical imaging monocytes/macrophages migration and activation in contact hypersensitivity (Conference Presentation)

Zhihong Zhang

Author Affiliations +

Proceedings Volume 10065, Biophotonics and Immune Responses XII; 100650D (2017) https://doi.org/10.1117/12.2251088
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Inflammatory monocytes/macrophages (Mon/Mφ) play an important role in cutaneous allergic inflammation. However, their migration and activation in dermatitis and how they accelerate the inflammatory reaction are largely unknown. Optical molecular imaging is the most promising tool for investigating the function and motility of immune cells in vivo. We have developed a multi-scale optical imaging approach to evaluate the spatio-temporal dynamic behavior and properties of immune cells from the whole field of organs to the cellular level at the inflammatory site in delayed type hypersensitivity reaction. Here, we developed some multi-color labeling mouse models based on the endogenous labeling with fluorescent proteins and the exogenous labeling with fluorescent dyes. We investigated the cell movement, cell interaction and function of immunocytes (e.g. Mon/Mφ, DC, T cells and neutrophils) in the skin allergy inflammation (e.g., contact hypersensitivity) by using intravital microscopy. The long-term imaging data showed that after inflammatory Mon/Mφ transendothelial migration in dermis, they migrating in interstitial space of dermis. Depletion of blood monocyte with clodronate liposome extremely reduced the inflammatory reaction. Our finding provided further insight into inflammatory Mon/Mφ mediating the inflammatory cascade through functional migration in allergic contact dermatitis.

Conference Presentation

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Zhihong Zhang "Dynamical optical imaging monocytes/macrophages migration and activation in contact hypersensitivity (Conference Presentation)", Proc. SPIE 10065, Biophotonics and Immune Responses XII, 100650D (19 April 2017); https://doi.org/10.1117/12.2251088

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KEYWORDS

Optical imaging

Inflammation

Blood

Fluorescent proteins

In vivo imaging

Microscopy

Molecular imaging

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