Blue LED induced thermal effects in wound healing: experimental evidence in an in vivo model of superficial abrasions

Francesca Rossi; Riccardo Cicchi; Giada Magni; Francesca Tatini; Stefano Bacci; Gaia Paroli; Domenico Alfieri; Cristina Tripodi; Gaetano De Siena; Francesco S. Pavone; Roberto Pini

doi:10.1117/12.2251947

22 February 2017 Blue LED induced thermal effects in wound healing: experimental evidence in an in vivo model of superficial abrasions

Francesca Rossi, Riccardo Cicchi, Giada Magni, Francesca Tatini, Stefano Bacci, Gaia Paroli, Domenico Alfieri, Cristina Tripodi, Gaetano De Siena, Francesco S. Pavone, Roberto Pini

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Proceedings Volume 10066, Energy-based Treatment of Tissue and Assessment IX; 100660B (2017) https://doi.org/10.1117/12.2251947
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

A faster healing process was observed in superficial skin wounds after irradiation with a blue LED (EmoLED) photocoagulator. EmoLED is a compact handheld device, used to induce a thermal effect and thus coagulation in superficial abrasions. We present the results of an in vivo study, conducted in a mouse model, to analyze the induced wound healing. Two superficial abrasions were produced on the back of the mice: one area was treated with EmoLED (1.4 W/cm2, 30 s treatment time), while the other one was left naturally recovering. During the treatment, a temperature around 40-45°C was induced on the abrasion surface. Mice back healthy skin was used as a control. The animals underwent a follow up study and were sacrificed at 0, 1, 3, 6, 9, 12, 18, 21, 24 hours p.o. and 6 days p.o.. Samples from the two abraded areas were harvested and examined by histopathological and immunofluorescence analysis, SHG imaging and confocal microscopy. The aim of the study was to investigate the inflammatory infiltrate, mastocyte population, macrophage subpopulation, fibroblasts and myofibroblasts. Our results show that soon after the treatment, both the inflammatory infiltrate and the M1 macrophage subpopulation appear earlier in the treated, compared to a delayed appearance in the untreated samples. There was no alteration in collagen morphology in the recovered wound. This study confirms the preliminary results obtained in a previous study on a rat model: the selective photothermal effect we used for inducing immediate coagulation in superficial wounds seems to be associated to a faster and improved healing process.

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Francesca Rossi, Riccardo Cicchi, Giada Magni, Francesca Tatini, Stefano Bacci, Gaia Paroli, Domenico Alfieri, Cristina Tripodi, Gaetano De Siena, Francesco S. Pavone, and Roberto Pini "Blue LED induced thermal effects in wound healing: experimental evidence in an in vivo model of superficial abrasions", Proc. SPIE 10066, Energy-based Treatment of Tissue and Assessment IX, 100660B (22 February 2017); https://doi.org/10.1117/12.2251947

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KEYWORDS

Wound healing

Skin

Blue light emitting diodes

Collagen

Second-harmonic generation

Thermal effects

In vivo imaging

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