Investigating mechanically induced phase response of the tissue by using high-speed phase-resolved optical coherence tomography (Conference Presentation)

Yuye Ling; Christine P. Hendon

doi:10.1117/12.2252376

24 April 2017 Investigating mechanically induced phase response of the tissue by using high-speed phase-resolved optical coherence tomography (Conference Presentation)

Yuye Ling, Christine P. Hendon

Author Affiliations +

Proceedings Volume 10067, Optical Elastography and Tissue Biomechanics IV; 100670Q (2017) https://doi.org/10.1117/12.2252376
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Phase-resolved optical coherence tomography (OCT), a functional extension of OCT, provides depth-resolved phase information with extra contrast. In cardiology, changes in the mechanical properties have been associated with tissue remodeling and disease progression. Here we present the capability of profiling structural deformation of the sample in vivo by using a highly stable swept source OCT system The system, operating at 1300 nm, has an A-line acquisition rate of 200 kHz. We measured the phase noise floor to be 6.5 pm±3.2 pm by placing a cover slip in the sample arm, while blocking the reference arm. We then conducted a vibrational frequency test by measuring the phase response from a polymer membrane stimulated by a pure tone acoustic wave from 10 kHz to 80 kHz. The measured frequency response agreed with the known stimulation frequency with an error < 0.005%. We further measured the phase response of 7 fresh swine hearts obtained from Green Village Packing Company through a mechanical stretching test, within 24 hours of sacrifice. The heart tissue was cut into a 1 mm slices and fixed on two motorized stages. We acquired 100,000 consecutive M-scans, while the sample is stretched at a constant velocity of 10 um/s. The depth-resolved phase image presents linear phase response over time at each depth, but the slope varies among tissue types. Our future work includes refining our experiment protocol to quantitatively measured the elastic modulus of the tissue in vivo and building a tissue classifier based on depth-resolved phase information.

Conference Presentation

Citation Download Citation

Yuye Ling and Christine P. Hendon "Investigating mechanically induced phase response of the tissue by using high-speed phase-resolved optical coherence tomography (Conference Presentation)", Proc. SPIE 10067, Optical Elastography and Tissue Biomechanics IV, 100670Q (24 April 2017); https://doi.org/10.1117/12.2252376

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Optical coherence tomography

Tissues

Phase measurement

Tissue optics

Heart

In vivo imaging

Acoustics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years