16 February 2017 Ultrafast, laser-scanning time-stretch microscopy with visible light
Author Affiliations +
We demonstrate ultrafast time-stretch microscopy in, to the best of our knowledge, the shortest wavelength regimes, i.e. 532 nm. This is enabled by a new all-optical ultrahigh-speed laser-scanning technique called free-space angular-chirpenhanced delay (FACED) that achieves a line-scan rate as high as 20 MHz. In contrast to the predominant fiber-based implementation, time-stretch imaging based on FACED allows wavelength-independent and low-loss operations, and more intriguingly reconfigurable all-optical laser-scanning rate. Using this technique, we present high-resolution single-cell images captured in an ultrafast microfluidic flow (1.5m/s). This could unleash numerous cell and tissue imaging applications, e.g. high-throughput image flow cytometry and whole-slide imaging.
Conference Presentation
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Wenwei Yan, Wenwei Yan, Jianglai Wu, Jianglai Wu, Kenneth K. Y. Wong, Kenneth K. Y. Wong, Kevin K. Tsia, Kevin K. Tsia, } "Ultrafast, laser-scanning time-stretch microscopy with visible light", Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 100681C (16 February 2017); doi: 10.1117/12.2251094; https://doi.org/10.1117/12.2251094

Back to Top