Spheroid imaging of phase-diversity homodyne OCT

Naoko Senda; Kentaro Osawa

doi:10.1117/12.2249715

16 February 2017 Spheroid imaging of phase-diversity homodyne OCT

Naoko Senda, Kentaro Osawa

Proceedings Volume 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV; 100681I (2017) https://doi.org/10.1117/12.2249715
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Non-invasive 3D imaging technique is essential for regenerative tissues evaluation. Optical coherence tomography (OCT) is one of 3D imaging tools with no staining and is used extensively for fundus examination. We have developed Phase-Diversity Homodyne OCT which enables cell imaging because of high resolution, whereas conventional OCT was not used for cell imaging because of low resolution. We demonstrated non-invasive imaging inside living spheroids with Phase-Diversity Homodyne OCT. Spheroids are spheroidal cell aggregates and used as regenerative tissues. Cartilage cells were cultured in low-adhesion 96-well plates and spheroids were manufactured. Cell membrane and cytoplasm of spheroid were imaged with OCT.

Citation Download Citation

Naoko Senda and Kentaro Osawa "Spheroid imaging of phase-diversity homodyne OCT", Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 100681I (16 February 2017); https://doi.org/10.1117/12.2249715

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KEYWORDS

Optical coherence tomography

Homodyne detection

Cartilage

Image resolution

Tissues

Objectives

Light scattering

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