16 February 2017 Rapid measurement of meat spoilage using fluorescence spectroscopy
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Abstract
Food spoilage is mainly caused by microorganisms, such as bacteria. In this study, we measure the autofluorescence in meat samples longitudinally over a week in an attempt to develop a method to rapidly detect meat spoilage using fluorescence spectroscopy. Meat food is a biological tissue, which contains intrinsic fluorophores, such as tryptophan, collagen, nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) etc. As meat spoils, it undergoes various morphological and chemical changes. The concentrations of the native fluorophores present in a sample may change. In particular, the changes in NADH and FAD are associated with microbial metabolism, which is the most important process of the bacteria in food spoilage. Such changes may be revealed by fluorescence spectroscopy and used to indicate the status of meat spoilage. Therefore, such native fluorophores may be unique, reliable and nonsubjective indicators for detection of spoiled meat. The results of the study show that the relative concentrations of all above fluorophores change as the meat samples kept in room temperature (~19° C) spoil. The changes become more rapidly after about two days. For the meat samples kept in a freezer (~-12° C), the changes are much less or even unnoticeable over a-week-long storage.
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Binlin Wu, Binlin Wu, Kevin Dahlberg, Kevin Dahlberg, Xin Gao, Xin Gao, Jason Smith, Jason Smith, Jacob Bailin, Jacob Bailin, } "Rapid measurement of meat spoilage using fluorescence spectroscopy", Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 1006820 (16 February 2017); doi: 10.1117/12.2253526; https://doi.org/10.1117/12.2253526
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